Doccol MCAO線(xiàn)拴可以將腦梗塞體積標準差減少至5%
成功的MCAO模型的腦血流在插入線(xiàn)拴時(shí)會(huì )有斷崖式下降；在拔出線(xiàn)拴后，激光多普勒檢測的血流會(huì )很快恢復（圖A）。采用硬質(zhì)尼龍魚(yú)線(xiàn)線(xiàn)拴，極易戳破血管，造成蛛網(wǎng)膜下腔出血（Subarachnoid hemorrhage，圖B）。手術(shù)過(guò)程中血管如果被線(xiàn)拴戳破，腦血流不會(huì )有斷崖式下降，而是緩慢下降；在拔出線(xiàn)拴后，腦血流也不會(huì )恢復。造成腦梗塞體積變異的原因還有，不完全的梗塞血管或線(xiàn)拴移位造成的血流下降程度不均***及提前灌注。因此，線(xiàn)栓的質(zhì)地是MCAO模型穩定的關(guān)鍵。
對于MCAO模型來(lái)說(shuō)，主要有兩種線(xiàn)拴，無(wú)包被的火焰熔燙圓頭線(xiàn)拴及硅膠包被線(xiàn)拴。研究表明(Tsuchiya et al. 2003)，無(wú)包被的圓頭線(xiàn)拴在MCAO實(shí)驗過(guò)程中可能導致高達40%的蛛網(wǎng)膜下腔出血，而其標準差可能比平均值的50%還要大。另***項技術(shù)文章表明(Shimamura et al. 2006a)，硅膠包被線(xiàn)拴要遠好于無(wú)包被的圓頭線(xiàn)拴，其結果更為穩定，即使對于沒(méi)有經(jīng)驗的實(shí)驗者來(lái)說(shuō)也是如此。而多聚賴(lài)氨酸包被的線(xiàn)拴只能增加死亡率，不能減少梗賽體積的變異程度(Huang et al. 1998)。
Doccol MCAO線(xiàn)拴全部為硅膠包被，圓潤、柔軟、包被均勻，即使在沒(méi)有激光多普勒實(shí)時(shí)檢測的情況，也不會(huì )將血管戳破。使用自制的硅膠包被的線(xiàn)拴，大鼠(Schmid-Elsaesser et al. 1998)及小鼠(Shah et al. 2006)的腦梗塞體積的標準差均在30%左右。而使用Doccol MCAO線(xiàn)拴可以得到更好的結果。對于大鼠MCAO模型來(lái)說(shuō)，使用Doccol MCAO線(xiàn)拴得到的結果的標準差可達10%-20% （Ruscher et al.2012；Ishizaka et al.2013；Sakata et al.2012；Candelario-Jalil et al. 2008；Khan et al. 2006；Liu et al. 2006；Shimamura et al. 2006b；Solaroglu et al. 2006；Tsubokawa et al. 2007；Tsubokawa et al. 2006a；Tsubokawa et al. 2006b)。對于小鼠來(lái)說(shuō)，使用Doccol MCAO線(xiàn)拴得到的結果的標準差可達5%-10%(Chen et al.2014; Bae et al.2013; Jin et al.2011; Gu et al.2012; Kleinschnitz et al. 2007; Maysami et al. 2008; Pignataro et al. 2007b; Pignataro et al. 2007c)。
References
1. Bae ON, Serfozo K, Baek SH, et al. (2013) Safety and efficacy evaluation of carnosine, an endogenous neuroprotective agent for ischemic stroke. Stroke 44(1):205-12.
2. Candelario-Jalil E, Munoz E, Fiebich BL. (2008) Detrimental effects of tropisetron on permanent ischemic stroke in the rat. BMC Neurosci 9:19
3. Chen HZ, Guo S, Li ZZ, et al. (2014) A critical role for interferon regulatory factor 9 in cerebral ischemic stroke.J Neurosci.34(36):11897-912.
4. Huang J, Kim LJ, Poisik A, Pinsky DJ, Connolly ES, Jr. (1998) Does poly-L-lysine coating of the middle cerebral artery occlusion suture improve infarct consistency in a murine model? J Stroke Cerebrovasc Dis 7:296-301
5. Gu L, Xiong X, Zhang H, et al. (2012) Distinctive Effects of T Cell Subsets in Neuronal Injury Induced by Cocultured Splenocytes In Vitro and by In Vivo Stroke in Mice. Stroke 43:1941-1946.
6. Ishizaka S, Horie N, Satoh K, et al. (2013) Intra-arterial Cell Transplantation Provides Timing-Dependent Cell Distribution and Functional Recovery After Stroke. Stroke 44:720-726.
7. Jin R, Song Z, Yu S, et al. (2011) Phosphatidylinositol-3-kinase gamma plays a central role in blood-brain barrier dysfunction in acute experimental stroke.Stroke 42(7):2033-44.
8. Khan M, Jatana M, Elango C, Paintlia AS, Singh AK, Singh I. (2006) Cerebrovascular protection by various nitric oxide donors in rats after experimental stroke. Nitric Oxide 15:114-124
9. Kleinschnitz C, Pozgajova M, Pham M, Bendszus M, Nieswandt B, Stoll G. (2007) Targeting platelets in acute experimental stroke: impact of glycoprotein Ib, VI, and IIb/IIIa blockade on infarct size, functional outcome, and intracranial bleeding. Circulation 115:2323-2330
10. Koizumi J, Yoshida Y, Nakazawa T, Ooneda G. (1986) Experimental studies of ischemic brain edema, I: a new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 8:1-8
11. Liu S, Liu W, Ding W, Miyake M, Rosenberg GA, Liu KJ. (2006) Electron paramagnetic resonance-guided normobaric hyperoxia treatment protects the brain by maintaining penumbral oxygenation in a rat model of transient focal cerebral ischemia. J Cereb Blood Flow Metab 26:1274-1284
12. Longa EZ, Weinstein PR, Carlson S, Cummins R. (1989) Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20:84-91
13. Maysami S, Lan JQ, Minami M, Simon RP. (2008) Proliferating progenitor cells: a required cellular element for induction of ischemic tolerance in the brain. J Cereb Blood Flow Metab 28:1104-1113
14. Pignataro G, Simon RP, Boison D. (2007a) Transgenic overexpression of adenosine kinase aggravates cell death in ischemia. J Cereb Blood Flow Metab 27:1-5
15. Pignataro G, Simon RP, Xiong ZG. (2007b) Prolonged activation of ASIC1a and the time window for neuroprotection in cerebral ischaemia. Brain 130:151-158
16. Pignataro G, Studer FE, Wilz A, Simon RP, Boison D. (2007c) Neuroprotection in ischemic mouse brain induced by stem cell-derived brain implants. J Cereb Blood Flow Metab 27:919-927
17. Ruscher K, Kuric E, Wieloch T, et al. (2012) Levodopa Treatment Improves Functional Recovery After Experimental Stroke. Stroke 43:507-513.
18. Sakata H, et al. (2012) Minocycline-Preconditioned Neural Stem Cells Enhance Neuroprotection after Ischemic Stroke in Rats. J Neurosci. 32(10):3462–3473.
19 Schmid-Elsaesser R, Zausinger S, Hungerhuber E, Baethmann A, Reulen HJ. (1998) A critical reevaluation of the intraluminal thread model of focal cerebral ischemia: evidence of inadvertent premature reperfusion and subarachnoid hemorrhage in rats by laser-Doppler flowmetry. Stroke 29:2162-2170
20. Shah ZA, Namiranian K, Klaus J, Kibler K, Dore S. (2006) Use of an optimized transient occlusion of the middle cerebral artery protocol for the mouse stroke model. J Stroke Cerebrovasc Dis 15:133-138
21. Shimamura N, Matchett G, Tsubokawa T, Ohkuma H, Zhang J. (2006a) Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model. J Neurosci Methods 156:161-165
22. Shimamura N, Matchett G, Yatsushige H, Calvert JW, Ohkuma H, Zhang J. (2006b) Inhibition of integrin alphavbeta3 ameliorates focal cerebral ischemic damage in the rat middle cerebral artery occlusion model. Stroke 37:1902-1909
23. Solaroglu I, Tsubokawa T, Cahill J, Zhang JH. (2006) Anti-apoptotic effect of granulocyte-colony stimulating factor after focal cerebral ischemia in the rat. Neuroscience 143:965-974
24. Tsubokawa T, Jadhav V, Solaroglu I, Shiokawa Y, Konishi Y, Zhang JH. (2007) Lecithinized superoxide dismutase improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats. Stroke 38:1057-1062
25. Tsubokawa T, Solaroglu I, Yatsushige H, Cahill J, Yata K, Zhang JH. (2006a) Cathepsin and calpain inhibitor E64d attenuates matrix metalloproteinase-9 activity after focal cerebral ischemia in rats. Stroke 37:1888-1894
26. Tsubokawa T, Yamaguchi-Okada M, Calvert JW, Solaroglu I, Shimamura N, Yata K, Zhang JH. (2006b) Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats. J Neurosci Res 84:832-840
27. Tsuchiya D, Hong S, Kayama T, Panter SS, Weinstein PR. (2003) Effect of suture size and carotid clip application upon blood flow and infarct volume after permanent and temporary middle cerebral artery occlusion in mice. Brain Res 970:131-139
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